## ----setup, include=FALSE-----------------------------------------------------
knitr::opts_chunk$set(echo = TRUE)
library(tidyverse)
library(phyloseq)

## -----------------------------------------------------------------------------
samples <- get0("samples", envir = asNamespace("FLORAL"))
counts <- get0("counts", envir = asNamespace("FLORAL"))
taxonomy <- get0("taxonomy", envir = asNamespace("FLORAL"))

phy <- phyloseq(
  sample_data(samples %>% column_to_rownames("SampleID")),
  tax_table(taxonomy %>% select(ASV, Kingdom:Genus) %>% column_to_rownames("ASV") %>% as.matrix()),
  otu_table(counts  %>% pivot_wider(names_from = "SampleID", values_from = "Count", values_fill = 0) %>% column_to_rownames("ASV") %>% as.matrix(), taxa_are_rows = TRUE)
) %>%  subset_samples(DayRelativeToNearestHCT > -30 & DayRelativeToNearestHCT < 0) %>% 
  tax_glom("Genus")

## -----------------------------------------------------------------------------
dat <- FLORAL::phy_to_floral_data(phy, covariates=c("Consistency"), y = "DayRelativeToNearestHCT")

## -----------------------------------------------------------------------------
res <- FLORAL::FLORAL(y = dat$y, x = dat$xcount, ncov = dat$ncov, family = "gaussian", ncv=NULL, progress=FALSE)